Application

Anti-MSL-2 Antibody, clone 10C3.1 is an antibody against MSL-2 for use in Western Blotting, Immunocytochemistry.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryUbiquitin & Ubiquitin Metabolism

Western Blotting Analysis: 2.0 µg/mL of this antibody detected MSL-2 in 10 µg of HepG2 and HEK293 cell lysates.
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected MSL-2 in HUVECs, A431 and HeLa cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

E3 ubiquitin-protein ligase MSL2 (UniProt Q9HCI7; also known as Male-specific lethal-2 homolog, Male-specific lethal-2 homolog 1, Male-specific lethal 2-like 1, MSL-2, MSL2-like 1, RING finger protein 184) is encoded by the MSL2 (also known as KIAA1585, MSL2L1, RNF184) gene (Gene ID 55167) in human. MSL2 (male-specific lethal 2) was originally identified in Drosophila and characterized as a gene whose mution causes male-specific lethality. Human MSL (hMSL) orthologues are found in an evolutionary conserved complex known as the hMOF (Males absent on the first, human) complex that is composed of hMOF, hMSL1, hMSL2, hMSL3, and Nucleoprotein 153 (NUP153). MSL2 is a RING domain-containing E3 ligase that targets the tumor suppressor p53 Lys351 for monoubiquitination, exposing a nuclear export motif within p53 as well as releasing p53 from MDM2. MSL2 overexpression causes an upregulation of mitochondrial-dependent apoptosis as a result of p53 cytoplasmic accumulation. hMSL1/hMSL2 heterodimer is reported to ubiquitinate histone H2B on Lys34 (H2BK34ub), which promotes transcription activation by stimulating the activities of MLL/SET1 family H3K4 methyltransferases and the H3K79 methyltransferase DOT1L. In addition, both hMSL1 and hMOF are reported to be modified by hMSL2 following DNA damage induction, and hMSL2-depletion causes impaired non-homologous end joining (NHEJ) repair in human cells.

Immunogen

Epitope: Internal (C-terminal half).

GST-tagged recombinant human MSL-2 C-terminal fragment.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in LNCaP cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected MSL-2 in 10 µg of LNCaP cell lysate.

Specificity

Expected to react with both spliced isoforms of human MSL2 reported by UniProt (Q9HCI7).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~62 kDa observed. 62.54 kDa (isoform 1) and 54.34 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.